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Taken together, we show that SLC25A11 can directly bind to CUL3, regulate the neddylation level of CUL3

Cell culture and transfection/electroporation 293 T human embryonic kidney cells were cultured in Dulbeccos modified Eagles medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, 100 units/mL of penicillin, and 100 g/mL of streptomycin and then transfected with expression vectors via the calcium phosphate-DNA precipitation method 33

Allow the alcohol to evaporate completely before proceeding to prevent chemical interference with the peptide

The 24-h UTP, serum scr and serum BUN concentrations of mice were detected with the specified detection kit

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6.1 Regulation of reproductive-related hormones The HPO axis is a highly coordinated neuroendocrine system integrating the hypothalamus, pituitary gland, and ovaries to regulate reproductive cyclicity