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The membrane was blocked with 3% non-fat milk (BioRad, Hercules, CA, United States) at room temperature for 1.5 h, washed, and incubated with primary antibodies (Supplementary Table 3) at 4C overnight on a rotating shaker

This inflammatory state not only damages surrounding healthy cells but can also lead to excessive activation of the immune system, further exacerbating tissue damage (Byrns China et al., 2024

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For example, the senescence of cardiac endothelial cells may exacerbate myocardial ischemia and heart failure