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Before examination, mice were anesthetized with an intraperitoneal injection of sodium pentobarbital (87.5 mg/kg) and the pupils were dilated with a mixed solution of 0.5% phenylephrine and 0.5% tropicamide

Your doctor will usually focus on the following factors: Examining the welts: Noting their size, colour, and how they move around the body Talking through your history: When the hives started, how long they last, and how often they appear Looking for triggers: Recent foods, new medications or supplements, environmental exposures, or stress Further tests arent usually needed unless the hives are persistent, uncomfortable, or come with other worrying signs, such as swelling of the lips, tongue, or throat

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The elution was carried out at a flow rate of 1 mL min 1