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10.1016/j.jbc.2021.101544 24 DumoreN

J Antimicrob Chemother 70 , 27972809 (2015)

The first two stages are integrated into the methionine cycle, while the subsequent two phases are integral components of the transsulfuration pathway

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1-3, were seeded at 20,000 onto 35mm poly-L-lysine-coated glass-bottom dishes (Celvis) or coverslips in Neurobasal growth medium supplemented with 2% B27, 2 mM Glutamax, 50 U/ml penicillin, 50 g/ml streptomycin, and 5% fetal bovine serum (FBS) (Wang et al