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Phosphopeptides were eluted in three rounds with 80 l 1% ammonia for 20 min, transfered the supernatant to a clean plate, and acidified with TFA

yellow asterisk in (figure 2.13) and CRYAB-HIS (blue asterisk in figure 2.13)

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This impaired clearance would also be compounded by exogenous copper downregulating LRP1 expression (Singh et al., 2013), that could further impede CNS clearance of LRP1 ligands such as Tau or A

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