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(iii) Most importantly, TMAO may be just one of many factors that influence atherosclerosis and its complications

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Constructions expressing the Wild type PKCI (Wt PKCI) and PKCI containing a deletion of the first thirty amino acids of the N-terminus, including the pseudo-substrate (NPS PKCI) were both cloned in the mammalian expression vector pcDNA 3.1 in Bam HI and Xho I sites using the following primers: forward 5 ATATTTAGGATCCCAGATGGCTGACCCG 3/reverse 5 CAGGTCGGTCTCGAGCTACACATTAATGAC 3 and forward 5 A TATTTAGGATCCCAGATGAACGTGCACGAG 3 reverse 5 CAGGTCGGTCTCGAGCTACACATTAATGAC 3, respectively

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doi: 10.1080/00071668.2017.1362688 140