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The vector for protein production, pJB-GST-HTS-HS-GFP, was created by first cloning a 7 amino acid spacer fragment containing an XhoI site into pET-15b (Novagen) using sticky ligation and BamHI/XhoI sites

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If CSI was offered first, or with rehab, did this CSI limit ability to achieve a patient acceptable symptom state, resulting in return for further CSI, further increasing chances of tissue deterioration and reducing chances of success of conservative care

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