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The AAR was excised from the heart and disrupted in 2 ml of ice-cold isolation buffer (250 mM sucrose, 10 mM HEPES, and 1 mM EGTA, pH 7.4) using a TissueRuptor 22

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Long-term METH use increases H3 acetylation in the brain, decreasing H3K9ac and H3K18ac while increasing H4K5 and H4K8 acetylation over time