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As a matter of fact, ALA is able to prevent the upregulation of intercellular adhesion-molecule-1 (ICAM-1) and vascular cell adhesion-molecule-1 (VCAM-1) in tumor necrosis factor (TNF-)-stimulated brain and aortic endothelial cells in vitro [33,35]

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The PCR products were gel-purified and subjected to bulk Sanger-sequencing

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