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The overall conformation of the GHSR-G i complex in our structures highly resembles the canonical state of the NTSR1-G i complex

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The sections were then washed with PBS, incubated with peroxidase-labeled secondary antibodies (Absin, Shanghai, China, 1:1000) at 37 C for 2 h, and washed with PBS

Even if it looks intact, inspect the liquid for glass dust or cloudiness