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Finally, the purified enzyme was concentrated to approximately 200 M, aliquoted into 20 l volumes in PCR tubes, flash-frozen in liquid nitrogen, and stored at -80 C

10.1155/2011/937861 54 IacobelliS.GuignardJ

2.10 Data analysis The results were expressed as the mean standard error of at least three independent experiments, using samples from different animals for each experiment

118, S23-S31S31

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Yusheng Shu, [email protected] Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers