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The cell viability assay was evaluated by using reagents MTS assay (MTS) by removing the cell supernatants and replacing with 120 L of MTS stock solution containing DMEM medium without phenol red (31.5 mL), MTS (2 mg/mL, 6 mL), PMS (0.92 mg/mL, 0.3 mL), incubation for 1 h and measurement of absorbance at 490 nm

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Bilirubin glucuronide formation in vitro : demonstration of a defect in Gilbert's disease

124 Martnez-ClarosM.SteinbuschH

J Affect Disord 135:38994

A moisturizer can be applied before or after to buffer ('sandwich' method)