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UMN culture is an important tool to investigate UMN responses to treatment in vitro UCHL-eGFP is a reporter mouse line for corticospinal motor neurons (CSMN, a.k.a the UMNs in mice), in which CSMN express eGFP, and CSMN identity of eGFP+ neurons in the layer 5 of the motor cortex is confirmed by retrograde labeling, molecular marker expression and electrophysiological properties 31

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Internalization and toxicity: a preliminary study of effects of nanoplastic particles on human lung epithelial cell

2.8 Permeability to lactulose The protocol for measuring monolayer permeability to lactulose was adapted from (37)

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Proc Natl Acad Sci USA 104: 2093220937 [DOI] [PMC free article] [PubMed] [Google Scholar] Benvenuti S., Lazzari L., Arnesano A., Li Chiavi G., Gentile A., Comoglio P.M