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When the light signal is positive, the SCN secretes gamma-amino butyric acid, responsible for the inhibition of the neurons that synapse in the paraventricular nucleus (PVN) of the hypothalamus, consequently the signal to the pineal gland is interrupted and melatonin is not synthesized

The resulting rankings, along with the gene lists, were then put into the function AUCell_calcAUC (aucMaxRank set to 100% of the number of input genes)

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But their compact sequences mean fewer vulnerable residues and less structural complexity to maintain