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Luminescence signal was quantified via a Varioskan Lux plate reader (ThermoFisher Scientific)

10.1186/s13020-023-00843-4 60 MaY.ZhaoY.LuoM.JiangQ.LiuS.JiaQ.et al (2024)

Gastroenterology 158 , 19992014.e1 (2020)

T-loop-phosphorylated Aurora-A is produced almost exclusively at centrosomes, while the high affinity of TPX2 for this kinase pool ensures that active Aurora-A:TPX2 complexes are present only on the spindle apparatus