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Description

Quantification of metabolites by LC-MS/MS To quantify polar metabolites concentrations, we used TSQ Vantage LC-MS interfaced with Ultimate 3000 Liquid Chromatography system (Thermo Scientific) and Triple quadruple mass spectrometry to detect metabolites from tissues or cells

Exposing cerebral mitochondria to THC was reported to induce a ten-fold increase in free radical leak and increased H 2 O 2 production [40], while in epithelial cells, THC upregulated inflammation-related genes and downregulated antioxidant-related genes [34]

Moreover, it promotes mitochondrial damage via reactive oxygen species (ROS) accumulation [30] and synergizes with doxorubicin (DOX) to exert potent antitumor effects [31]