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Study Limitations Although this pilot exploratory study provides proof-of-concept that GlyNAC supplementation from a younger age can increase length of life, the main limitation is that the study was done in a relatively small number of mice, and data is not reported from every organ in the body

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Heterogeneity across the methods of different studies which include alterations in sample collection, data analysis, and sequencing techniques adds a further layer of complexity in drawing appropriate conclusions

For instance, MOLs enriched in the brain subventricular zone express a unique profile of transcription factors linked to development, while spinal cord-enriched MOLs are defined by a specific upregulation of immune-related gene signatures (Weng et al., 2025

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