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Moreover, HIF-1 appears to play a critical role in modulating salivary gland development and function under hypoxic conditions

As a superficial tumor, the causes of death in patients with breast cancer are reported to be due to cancer metastasis and/or relapse, which are significantly associated with the favorable tumor microenvironment [4]

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HEK293T cells were grown to 70% confluency in 96-well plates (Corning) at 37C in DMEM supplemented with 10% FBS, and treated with Entranster TM -H (Engreen) transfection reagent (0.1 L) and purified plasmids [0.25 g pGL4.74 (hRluc/TK) and 0.25 g pGL4.32(luc2P/NF-B-RE/Hygro plasmid)] in 50 L DMEM/10% FBS per well