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Immunohistochemistry analysis The colon samples were immediately fixed with 4% paraformaldehyde for 24 h upon separation, followed by paraffin embedding and cutting to a thickness of 4 m

Relative energy deficiency in sports (RED-S): elucidation of endocrine changes affecting the health of males and females

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Supplementary Table 6), known to inhibit OL generation

THP-1 cells, a human acute monocytic leukemic cell line, were obtained from ATCC (TIB-202) and cultured in an incubator at 37 C, 5% CO 2 , and 95% humidity in RPMI-1640 medium containing 2 mM L-glutamine, 25 mM HEPES, 1% sodium pyruvate, 0.01% of 2-mercaptoethanol (2-ME), 10% fetal bovine serum (FBS), and 1% penicillin/streptomycin

doi: 10.3389/fnmol.2021.691733 Received 07 April 2021 Accepted 06 August 2021 Published 31 August 2021 Volume 14 - 2021 Edited by Yong Chen, Duke University, United States Reviewed by Marc Surette, Universit de Moncton, Canada