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SIRT1: A potential therapeutic target in autoimmune diseases

Fluorescence polarization anisotropy assay Fluorescence polarization assays were conducted using a Cytation 5 cell imaging multi-mode reader (BioTek) equipped with a green FP filter at 25 C, utilizing 96-well microplates (SPL 30496)

Of the NTD-directed mAbs, Group B mAbs (WRAIR-5009, -5010, and -5013) were the only mAbs that neutralized WA-1, while showing no cross-neutralization to other SARS-CoV-2 VoC (Fig

The resin was boiled in SDS loading buffer (Bio-rad) and loaded on the SDS-PAGE