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Ozonized autohaemotransfusion and fibrinolytic balance in peripheral arterial occlusive disease

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Pantothenate synthetase (PS) Pantothenate (vitamin B5) is a necessary precursor for the biosynthesis of coenzyme A (CoA) and acyl carrier proteins (ACP)

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After the cross-linking reaction, 10 L of the reaction mixture was suspended into an equal volume of sample buffer for SDS-PAGE (Nacalai-Tesque) analysis, incubated for 3 min at 100 C, loaded onto a 12% SDS-PAGE gel, and the gel was stained by CBB stain One Super (Nacalai-Tesque)