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After 48 h, cells were washed with ice-cold PBS and lysed with 700 l of non-denaturing DISC lysis buffer (30 mM Tris-HCl (pH 7.4), 120 mM NaCl, 2 mM EDTA, 2 mM KCl, 1% Triton X-100, Roche complete protease inhibitor cocktail, 10 M PR619 and 1 mM NEM) on ice for 30 min

Hydrogen peroxide solution was substituted by phosphate buffer in the negative control

Muscle Preservation: Preserve your hard-earned muscle mass while shedding fat, ensuring a lean and toned physique with every workout

ALT is predominantly found in the liver, making it a more specific marker for liver damage

Repeat Injections May Be Needed for Long Term Pain Relief Patients who have a positive but incomplete response to a single injection may benefit from a repeat injection

The amino acid sequences of these polypeptides differ from the naturally occurring proteins corresponding to the markers encompassed by the present invention, but retain biological activity