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Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) Total miRNAs were isolated from the RIN-m5f cells using RNAiso Plus reagent (TaKaRa, Japan)

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The cellular commitment to protein quality control engages molecular strategies that include protein refolding via the chaperone network, protein degradation via the ubiquitin proteasome system, and aggregate elimination via autophagy, which work to cull misfolded proteins that otherwise might impair healthy cell functions 2

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10.1248/bpb.29.1597 21 ChangH

MiRNA is a small noncoding RNA molecule, 2122 nucleotides long, that promotes mRNA degradation by inhibiting translation or binding semi-complementarily to the 3 untranslated region (UTR), thereby regulating post-transcriptional gene expression 109,110