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However, there are few side effects that may occur as expected reaction of the body to the treatment such as redness, swelling and mild skin irritation

Mol Cell Biol 39(20):e00273-e319 Yu C, Xiao J-H (2021) The Keap1-Nrf2 system: a mediator between oxidative stress and aging

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Cells were incubated for 60 min at 37 C without CO 2 , pelleted by centrifugation for 5 min at 1200 g , washed 1x in 1 ml of ice-cold 1% BSA-DPBS, pelleted by centrifugation, resuspended in 300 l cell viability stain (1 M Calcein Violet 450 AM (CV450, eBioscience, San Diego, CA, USA) in DMEM-HI without supplements or FBS), incubated 15 min on ice for and analyzed by flow cytometry (Fortessa, BD, San Jose CA, USA) with 10 000 cellular events analyzed per sample, detecting DDAOG with 640 nm excitation and 670 nm emission, and CV450 with 405 nm excitation and 450 nm emission

ZnT3: a zinc transporter active in several organs