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At a pH of 7.4, primary nucleation proceeds spontaneously and is the fastest in the absence of other governing factors (Dada et al., 2023) whereas the critical concentration of -synuclein needed to undergo LLPS is the least at pH 5.5 probably owing to the proximity of the isoelectric point of -synuclein near that pH assisting separation of proteins from the solution (Hou et al., 2023)

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