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After cell lysis, the chromatin was fragmented into 100500 bp by Bioruptor Sonicator (Diagenode), and protein-DNA complexes were immunoprecipitated by 5 g HIF-1 antibody or 2 g anti-IgG antibody conjugated with Dynabeads Protein G (Invitrogen) mix on a rotator at 4 C overnight

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