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For the assay, 1.5 mL of the protein extract was mixed with 5 mL of Bradford dye reagent (0.01% Coomassie Brilliant Blue G-250 dissolved in 4.7% ethanol and 8.5% phosphoric acid)

However, caution is advised when combining hesperidin with certain medications, and caution is advised to prevent diminished efficacy or enhanced adverse effects

otorhinolaryngology 120, 3035

doi: 10.1016/j.jconrel.2014.02.002 204 PflanznerT.KuhlmannC

The RNS include: nitric oxide radical (NO ), nitroxyl anion (NO ), nitrosonium cation (NO + ) and peroxynitrite anions (ONOO ) (Halliwell, 2012

5G), suggesting that Sirt7 directly interacts with nucleosomes via the C-terminal basic region