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T cell purification, activation, differentiation and stimulation Purification, activation, differentiation and stimulation of CD8 + T cells or CD4 + T cells derived from C57BL/6 mice + + Nave CD8 + T cells or nave CD4 + T cells were isolated from spleens of C57BL/6 mice using a naive CD8 + T cell or naive CD4 + T cell isolation kit according to manufacturer instructions (BioLegend)

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Polyhistidine The most commonly used tag is the polyhistidine tag (Yip et al