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NAD+'s true power in energy metabolism becomes clear in the electron transport chain, where NADH turns back into NAD+ in complex I

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Methods: Male Wistar rats were subjected to a chow (CHOW) diet or a free-choice (fc) diet of either chow, saturated fat and liquid sugar (fcHFHS), chow and saturated fat (fcHF), or chow and liquid sugar (fcHS) for 4 weeks

For lipid reporter experiments, HEK293 cells were co-transfected with plasmids with AKT-PH::GFP and shRNA against KIAA1109 (Sigma-Aldrich, TRCN0000263343 with 73% knockdown efficiency), incubated for 48 h and imaged by confocal microscopy for membrane localized GFP

The refinement was carried out in several cycles of automated refinement in phenix.refine program [43] and manual model building performed in Coot [44]

The results obtained from the conducted experiments suggested that miR-181 variants played a role in regulating the activation of microglia