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Combinations of these factors contributing to low GPx1 activity/levels will result in lower capacity to respond to reactive oxygen species (ROS), favouring accumulation of oxidative damage and promoting tumour progression

Results Gpx3 is upregulated in BALF and tissue in the mouse model of bleomycin-induced pulmonary fibrosis Initially, in order to assess expression and regulation of lung-abundant extracellular antioxidant proteins, we extracted the relevant data from a previously published proteomic study on bleomycin-induced pulmonary fibrosis 35

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We also observed that 90% of the enzyme activity was lost when reactions were conducted in a DTT-containing (100 mM) reaction solution, likely because glycine oxidation cannot proceed and/or C281 is fully reduced (underscoring the essentiality of the post-translational modification) (Supplementary Table 3)