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Therefore, collectively, the functional efflux data and structural analysis provide conclusive evidence that P4S is not merely an orthosteric inhibitor but a translocatable substrate analog that competes with taurine for binding and undergoes the full Na-coupled transport cycle

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The table below shows these proportions: Chemical Ion Contributing to Seawater Salinity Concentration in o/oo (parts per thousand) in average seawater Proportion of Total Salinity (no matter what the salinity) Chloride 19.345 55.03 Sodium 10.752 30.59 Sulfate 2.701 7.68 Magnesium 1.295 3.68 Calcium 0.416 1.18 Potassium 0.390 1.11 Bicarbonate 0.145 0.41 Bromide 0.066 0.19 Borate 0.027 0.08 Strontium 0.013 0.04 Fluoride 0.001 0.003 Other less than 0.001 less than 0.001 The measurements listed in the table above from Castro and Huber's, Marine Biology textbook

Samples were incubated with GdmCl for 1 h before measurements

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