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High-throughput sequencing was performed with a MiSeq Illumina sequencer (2 300 bp) by the Translational Genomics (NGS) Unit of the Ramn y Cajal Hospital The Quantitative Insights into Microbial Ecology (QIIME2) pipeline (version qiime2-2020.8) was used to perform the quality filtering, discard chimeric sequences, merge the paired-end reads and estimate the Amplicon Single Variants (ASVs) 64

For example, altered levels of short-chain ACs, including C2 and C3, have been observed in coronary artery disease and diabetes [6, 55]

These estimates were based on one way ANOVA followed by the Student NewmanKeuls test

Naltrexone (TRMP3 variant): A 2019 cell study using CFS/ME cells from 8 different patients showed that Naltrexone restored TRPM3 function.[ref] Keep in mind that this is just a cell study (not a randomized, double-blind, placebo-controlled trial), but it may be worth talking with your doctor about this study, especially if you carry a TRPM3 variant linked to CFS

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