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The reaction buffer was prepared as follows: To 100 L potassium phosphate buffer (0.1 M, pH 7.0) we added 25 L of an 0.1 M aqueous EDTA solution, 25 L of aqueous sodium azide (10 mM), 25 L of an aqueous glutathione reductase solution (2.4 IU/ml), 25 L of an aqueous glutathione solution (0.01 M), 25 L of an NADPH solution (1.5 mM dissolved in sodium bicarbonate)