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500 ng recombinant human TLR4 (hTLR4) (Sino Biological Inc., Beijing, China) was coated onto 96-well immune plates (Thermo Fisher scientific), and nonspecific binding was blocked with 10 mM HEPES buffer (pH 7.4) containing 0.15 M NaCl, 5 mM CaCl 2 , and 2% fatty acid-free BSA (buffer A)

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Surface cleaning and duct decontamination may also be necessary to achieve a complete solution in the event of severe contamination

It was argued the solution would be to stop increasing taxes

The final lists of upregulated and downregulated proteins were then analyzed using DAVID

The introduction of excise duty on vaping products and the ban on disposable vapes reflect efforts to control the environmental and health impacts of vaping