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Human pancreatic stellate cell (hPSC) culture and treatments HPSCs (Fenghui Biotechnology Co., Ltd., Changsha, China) were cultured in RPMI 1640 medium (Gibco) with glucose (5.5 mmol/L glucose) supplemented with 10% FBS (Gibco) in an incubator with 5% CO2 at 37 C

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Based on the published literature on Nrf2, the rise of Nrf2 protein levels is likely due to impaired degradation of the Nrf2 protein, rather than an induction of its transcription or translation

I.NazeerA.et al

Another study has reported that metformin seemingly increases the production of 3-hydroxy fatty acids (3-HFAs) either by beta-oxidation or via gut microbiota

Inhalation drug delivery devices: technology update