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The UltiMate 3000 UHPLC system (Thermo Scientific) and EASY-Spray PepMap RSLC C18 50 cm 75 m ID column (Thermo Fisher Scientific) coupled with Orbitrap Fusion (Thermo) were used to separate fractionated peptides with a 530% acetonitrile gradient in 0.1% formic acid over 120 min at a flow rate of 250 nL/min

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Leston J, Harth C, Brun J, Mottolese C, Mertens P, Sindou M, et al