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Identification of inhibitors of protein-protein interactions has been a major challenge in drug discovery, yet screening strategies, including high-throughput screening and fragment-based drug design, are able to identify compounds suitable for drug development

The deletion of PNPLA2 in adipocytes resulted in reduced PPAR activity and expression of target genes, including lower UCP1 protein levels and when exposed to cold, mice were cold sensitive (140)

Hassannia B, Wiernicki B, Ingold I, Qu F, Van Herck S, Tyurina YY, et al

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After washing three times with PBS, anti-human IgG-Fc/APC was added and incubated for another 30 min

In addition, GSSG retained in mitochondria during oxidative stress can lead to the S-gluthionylation of target proteins with mitochondrial dysfunction (20, 21)