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Since NAc is also considered a part of striatum, we decided to use striatal tissues, which include NAc, for our co-immunoprecipitation experiment

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Statistical significance in a, g were determined by one-way ANOVA followed by Bonferroni post-hoc analysis

The cell lysates were solubilized in RIPA buffer containing 1 mM sodium orthovanadate, 1 mM sodium fluoride, 10 mM N-ethylmaleimide, 5 g/mL leupeptin, 5 g/mL aprotinin, and 2 mM phenylmethylsulphonyl fluoride, after which they were immunoprecipitated with FLAG beads

The tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu2+ stimulates matrix metalloproteinase-2 expression by fibroblast cultures