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Description

In total, 1 L of cDNA, together with 5 L of SYBR green mix (TIANGEN Biotech, Hainan, China), 0.3 L each forward and reverse primer, were added into the PCR mixtures (with the total volume of 10 L)

Subsequent research revealed that CUR increased ALP, COL1, and RUNX2 proteins and mRNA and ALP activity

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N-acetylcysteine N-acetylcysteine (NAC) is a modified form of L-cysteine and it is a widely used therapeutic agent, especially for mucolytic therapy and treating paracetamol overdose