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Lancet (2015) 386:98394

If youre someone who drinks daily, even moderate amounts, we talk about gradually reducing intake during prep week rather than stopping abruptly, which can cause rebound inflammation

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After three PBS washes, iHLOs were incubated in 100 L Ce3D permeabilization/blocking buffer (BioLegend, catalog: 427706) for 1 h at room temperature

Concentration: 5000 micrograms divided by 2 mL = 2500 micrograms per mL