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higher scores represent more active disease), an endoscopic subscore of 2, colonic involvement of 15 cm, and a confirmed diagnosis of ulcerative colitis for 3 months

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After qPCR quantification using the KAPA library quantification kit (Roche), Sequencing was carried out on the NovaSeq 6000 instrument from Illumina based on a 2 100 cycle mode (paired-end reads, 100 bases) to obtain around 100 million clusters (200 million raw paired-end reads) per sample

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