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Indeed, when GSAAT is reduced with gradually increasing amounts of DTT prior to incubation with NEM, either one Cys (Band 1, with 0.1 mM DTT) or no Cys residue of GSAAT is accessible for mPEG binding (with DTT 1 mM) ( Figure 2C )

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Three replicates were performed for each FACS experiment and 17,00028,000 cells were eventually included in statistical analysis (Fig

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Blood samples were centrifuged immediately after collection