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Finally, quantified real-time PCR (qPCR) analysis was conducted using TaqMan Gene Expression Assays ( RGMa : Mm00624998_m1, Neo1 : Gapdh: Mm99999915_g1, Thermo Fisher Scientific) as instructed by the manufacturer

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73 AkimotoTKumaiYAkamaTHayashiEMurakamiHSomaRet al

Taxonomic resolution at family level revealed the decrease in Firmicutes was mainly driven by a decrease in Lachnospiraceae ( P = 0.07) and the increase in Bacteroidota was primarily due to a significant increase in Prevotellaceae ( P = 0.015) (Fig