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Cells were grown in Terrific Broth (TB) medium at 37 C until reaching an optical density of 1.2 at 600 nm, then induced at 18 C for 16 h with 0.24 mM isopropyl--D-thiogalactoside (IPTG)

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Acknowledgments The authors thank to Unidad de Cmputo and Francisco Prez Eugenio of Instituto de Fisiologa Celular for computer facilities