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Cross-contamination between vials spreads problems

10.1371/journal.pone.0134050 127 SuenaertP.BulteelV.LemmensL.NomanM.GeypensB.Van AsscheG.et al (2002)

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The timepoint chosen (when drug-tolerant cells are emerging after treatment) probably contributes to this effect, and later timepoints (when cells perhaps enter senescence or other more terminally arrested states) are more likely to reflect the expected gene expression patterns