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After each injection of nanobody or Fab, the chip was regenerated using 3 M GuHCl (IBA Lifesciences) before re-immobilization of fresh gB protein in the respective channels

( e ) Growth curves of CAR-T cells generated using primary human T cells from healthy donors showed that most CAR-T cells and EGFRt control T cells grew after a complete medium change to RPMI medium supplemented with dFBS, IL-2, and IL-15 on day 0