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Western blotting Pancreatic cancer cells were lysed and the total protein was extracted with RIPA buffer (Beyotime, Guangzhou, China)

The lack of efficacy of inhaled GSH in patients with cystic fibrosis could be explained by the high concentrations of the GSH-degrading enzyme -glutamyltransferase present in lung fluids of those patients (116123), and then, the use of precursors of GSH synthesis like NAC and cystine could be more effective in the synthesis of GSH (124)

The area of the gaps was measured using ImageJ software for quantification

Peptide stability and delivery studies : The physicochemical properties of Dihexa, including its resistance to enzymatic degradation and favorable membrane permeability, are of particular interest in formulation science and peptide delivery research

Endocrinology 2013