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Briefly, 0.1 g leaves were extracted with 10 mL of 10% trichloroacetic acid (TCA) and then centrifuged at 10,000g for 20 min

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These used to be a commonly performed procedure as they can undoubtedly provide some pain relief, but the effect usually wears off within a few weeks and then the patient comes back wanting another one, and then another one

For intermediate precision of the method, the intraday and inter-day analysis was done, i.e., repeated the same concentrations six times a day at a gap of one hour each ( n = 6) and on 6 successive days, respectively