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2035-A DEVELOPMENT OF A NON-INVASIVE DIAGNOSIS FOR FATTY LIVER IN CHILE: PRELIMINARY RESULTS Maria Spencer Sandino 1,2 , Franco Godoy 1,2 , Claudio Vargas 3 , Felipe Elorrieta 4 , Laura Huidobro 2,5 , Francisco Barrera 6 and Catterina Ferreccio 1,2 , (1)Pontificia Universidad Catlica De Chile, (2)Advance Center for Chronic Diseases, Accdis, (3)Hospital Posta Central, (4)Universidad De Santiago De Chile, (5)Universidad Catlica Del Maule, (6)The University of Sydney Background: Non-alcoholic fatty liver disease (NAFLD) is the most frequent chronic liver disease

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Cells were then incubated with primary antibodies for 2 h and appropriate secondary antibodies Alexa Fluor 488 or 568 (ThermoFisher) for 1 h at room temperature

Infect Immun (2004) 72(5):262834