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The deproteinized samples were then diluted using lysis buffer, mixed with glutathione (GSH) and total glutathione (TGAM or GSH + GSSG) assay probes, incubated for 60 minutes protected from light, and fluorescence signal measured at Ex/Em = 490/520 nm using a BioTek Cytation 5 Multimode Reader

The presence of a variation in ERCC2 gene (rs13181 and rs1799793) reduces repair capacity, and results in greater efficacy of cisplatin treatment due to increased DNA damage and an enhanced cytotoxic effect

doi: 10.1002/path.5830 25 SinghAKPraharajMLombardoKAYoshidaTMatosoABarasASet al

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